Desmoglein-3 Antibody (1T1D5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16597

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1T1D5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Desmoglein-3 Antibodies »

Product Summary for Desmoglein-3 Antibody (1T1D5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 900-999 of human Desmoglein-3 (P32926). TLSGSQGASALSTSGSVQPAVSIPDPLQHGNYLVTETYSASGSLVQPSTAGFDPLLTQNVIVTERVICPISSVPGNLAGPTQLRGSHTMLCTEDPCSRLI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Desmoglein-3 Antibody (1T1D5)

Western Blot: Desmoglein-3 Antibody (1T1D5) [NBP3-16597]

Western Blot: Desmoglein-3 Antibody (1T1D5) [NBP3-16597]

Western Blot: Desmoglein-3 Antibody (1T1D5) [NBP3-16597] - Western blot analysis of extracts of various cell lines, using Desmoglein-3 Rabbit mAb (NBP3-16597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (1T1D5) [NBP3-16597]

Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (1T1D5) [NBP3-16597]

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (1T1D5) [NBP3-16597] - Immunofluorescence analysis of NIH-3T3 cells using Desmoglein-3 Rabbit mAb (NBP3-16597) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Desmoglein-3 Antibody (1T1D5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Desmoglein-3

Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

Alternate Names

CDHF6, Desmoglein3, DSG3

Gene Symbol

DSG3

Additional Desmoglein-3 Products

Product Documents for Desmoglein-3 Antibody (1T1D5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Desmoglein-3 Antibody (1T1D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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