Desmoglein-3 Antibody (5G11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1643

Novus Biologicals, part of Bio-Techne
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NB100-1643
NB100-1643SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Dot Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Cytometric Bead Assay Standard, IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G11

Format

BSA Free

Concentration

1 mg/ml

View all Desmoglein-3 Antibodies »

Product Specifications

Immunogen

Partial recombinant Human Desmoglein 3 protein expressed in baculovirus (amino acids 1-615)[UniProt# P32926]

Localization

Cell membrane; Single-pass type I membrane protein. Cell junction > desmosome

Specificity

This antibody reacts with Desmoglein-3. Does not cross-react with Desmoglein 1 or Desmoglein 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Desmoglein-3 Antibody (5G11) - BSA Free

Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5G11) - BSA Free [NB100-1643]

Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5G11) - BSA Free [NB100-1643]

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 in A-431 cells.
Immunohistochemistry: Desmoglein-3 Antibody (5G11) - BSA Free [NB100-1643]

Immunohistochemistry: Desmoglein-3 Antibody (5G11) - BSA Free [NB100-1643]

Immunohistochemistry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 on human skin cancer. NB100-1643 was used at a dilution of 1:200.
Flow Cytometry: Desmoglein-3 Antibody (5G11) - BSA Free [NB100-1643]

Flow Cytometry: Desmoglein-3 Antibody (5G11) - BSA Free [NB100-1643]

Flow Cytometry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Desmoglein-3 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Applications for Desmoglein-3 Antibody (5G11) - BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 15588331)

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Desmoglein-3

Desmoglein 3 (Dsg3) is a member of sub-family the desmoglein (DSGs) and it mainly serves as adhesion component of intercellular desmosome junctions. DSGs serve essential part of the core complex of desmosomes, and together with desmocollin (DSCs), they represents transmembrane proteins which mediate adhesion by transinteracting with cadherins of the opposing cell. Homophilic (e.g., DSG3-DSG3) as well as heterophilic (e.g., DSC3-DSG1) transinteraction have been reported for these proteins and within the desmosome, DSGs/DSCs are anchored to the intracellular plaque proteins plakoglobin, plakophilins, and desmoplakin, the latter of which mediates connection to the intermediate filament cytoskeleton. Desmoglein 3 is predominately expressed in stratified squamous epithelia including epidermis, tongue, tonsil, esophagus and carcinomas. Desmoglein 3 has been characterized as an auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease, wherein the binding of anti-desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Alternate Names

CDHF6, Desmoglein3, DSG3

Entrez Gene IDs

1830 (Human)

Gene Symbol

DSG3

UniProt

P32926

Additional Desmoglein-3 Products

Product Documents for Desmoglein-3 Antibody (5G11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Desmoglein-3 Antibody (5G11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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