Desmoglein-3 Antibody (5H10) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78984F

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP1-78984

Alexa Fluor 350

NBP1-78984AF350

Alexa Fluor 405

NBP1-78984AF405

Alexa Fluor 488

NBP1-78984AF488

Alexa Fluor 532

NBP1-78984AF532

Alexa Fluor 594

NBP1-78984AF594

Alexa Fluor 647

NBP1-78984AF647

Alexa Fluor 700

NBP1-78984AF700

Alexa Fluor 750

NBP1-78984AF750

Biotin

NBP1-78984B

CoraFluor 1

NBP1-78984CL1

DyLight 350

NBP1-78984UV

DyLight 405

NBP1-78984V

DyLight 488

NBP1-78984G

DyLight 550

NBP1-78984R

DyLight 594

NBP1-78984DL594

DyLight 650

NBP1-78984C

DyLight 680

NBP1-78984FR

DyLight 755

NBP1-78984IR

HRP

NBP1-78984H

Janelia Fluor 525

NBP1-78984JF525

Janelia Fluor 549

NBP1-78984JF549

Janelia Fluor 585

NBP1-78984JF585

Janelia Fluor 635

NBP1-78984JF635

Janelia Fluor 646

NBP1-78984JF646

Janelia Fluor 669

NBP1-78984JF669

mFluor Violet 450 SE

NBP1-78984MFV450

mFluor Violet 500 SE

NBP1-78984MFV500

mFluor Violet 610 SE

NBP1-78984MFV610

Forumulation

Catalog #

Azide and BSA Free

NBP2-80695

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # 5H10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Desmoglein-3 Antibodies »

Product Summary for Desmoglein-3 Antibody (5H10) [FITC]

Immunogen

The extracellular domain of human Desmoglein 3 [Swiss-Prot# P32926]

Localization

Cell membrane; Single-pass type I membrane protein. Cell junction > desmosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Desmoglein-3 Antibody (5H10) [FITC]

Application

Recommended Usage

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Desmoglein-3

Desmoglein 3 (Dsg3) is a member of sub-family the desmoglein (DSGs) and it mainly serves as adhesion component of intercellular desmosome junctions. DSGs serve essential part of the core complex of desmosomes, and together with desmocollin (DSCs), they represents transmembrane proteins which mediate adhesion by transinteracting with cadherins of the opposing cell. Homophilic (e.g., DSG3-DSG3) as well as heterophilic (e.g., DSC3-DSG1) transinteraction have been reported for these proteins and within the desmosome, DSGs/DSCs are anchored to the intracellular plaque proteins plakoglobin, plakophilins, and desmoplakin, the latter of which mediates connection to the intermediate filament cytoskeleton. Desmoglein 3 is predominately expressed in stratified squamous epithelia including epidermis, tongue, tonsil, esophagus and carcinomas. Desmoglein 3 has been characterized as an auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease, wherein the binding of anti-desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Alternate Names

CDHF6, Desmoglein3, DSG3

Gene Symbol

DSG3

Additional Desmoglein-3 Products

Product Documents for Desmoglein-3 Antibody (5H10) [FITC]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Desmoglein-3 Antibody (5H10) [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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