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Desmoplakin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49879

Novus Biologicals, part of Bio-Techne
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NBP1-49879

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-QESVKQAHLRNEH corresponding to internal region according to NP_004406.2.

Epitope

QESVKQAHLRNEH

Specificity

This antibody is expected to recognize reported isoform I (NP_004406.2) only.

Marker

Desmosomes Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Desmoplakin Antibody

Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879]

Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879]

Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879] - Paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Desmoplakin Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:4000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments in lysates of HEK293, HeLa and NIH3T3 gave no specific signal but low background. IHC-P: Human heart shows staining of the junctons between cardiomuscular fibres.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Desmoplakin

Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

Alternate Names

DCWHKTA, DPI, DPII, DSP, KPPS2, PPKS2

Entrez Gene IDs

1832 (Human); 109620 (Mouse); 306871 (Rat)

Gene Symbol

DSP

Additional Desmoplakin Products

Product Documents for Desmoplakin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Desmoplakin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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