Dfna5 Antibody (1E10)
Novus Biologicals, part of Bio-Techne | Catalog # H00001687-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1E10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DFNA5 (NP_004394.1, 111 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGN
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Dfna5 Antibody (1E10)
![Western Blot: Dfna5 Antibody (1E10) [H00001687-M01]](https://resources.bio-techne.com/images/products/Dfna5-deafness-Antibody-1E10-Western-Blot-H00001687-M01-img0002.jpg "Western Blot: Dfna5 Antibody (1E10) [H00001687-M01]")
Western Blot: Dfna5 Antibody (1E10) [H00001687-M01]
Western Blot: Dfna5 Antibody (1E10) [H00001687-M01] - Analysis of DFNA5 expression in transfected 293T cell line by DFNA5 monoclonal antibody (M01), clone 1E10.Lane 1: DFNA5 transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Dfna5 Antibody (1E10) [H00001687-M01] - Detection limit for recombinant GST tagged DFNA5 is 3 ng/ml as a capture antibody.
Applications for Dfna5 Antibody (1E10)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Dfna5
Alternate Names
deafness, autosomal dominant 5, DFNA5, deafness associated tumor suppressor, gasdermin-E, ICERE1, ICERE-1, Inversely correlated with estrogen receptor expression 1, nonsyndromic hearing impairment protein, non-syndromic hearing impairment protein 5
Entrez Gene IDs
1687 (Human)
Gene Symbol
GSDME
UniProt
Additional Dfna5 Products
Product Documents for Dfna5 Antibody (1E10)
Product Specific Notices for Dfna5 Antibody (1E10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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