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DGCR8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30115

Novus Biologicals, part of Bio-Techne
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NBP1-30115

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Synthetic peptide, which maps to a region between residue 1 and 50 of human DiGeorge syndrome critical region gene 8 using the numbering given in entry NP_073557.3

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGCR8 Antibody

Western Blot: DGCR8 Antibody [NBP1-30115]

Western Blot: DGCR8 Antibody [NBP1-30115]

Western Blot: DGCR8 Antibody [NBP1-30115] - Whole cell lysate from HeLa and 293T cells. DGCR8 was also IPed by rabbit anti-DGCR8 antibody NBP1-30116. For blotting immunoprecipitated DGCR8, the ReliaBLOT Reagents and Procedures were used.
Immunohistochemistry-Paraffin: DGCR8 Antibody [NBP1-30115]

Immunohistochemistry-Paraffin: DGCR8 Antibody [NBP1-30115]

Immunohistochemistry-Paraffin: DGCR8 Antibody [NBP1-30115] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DGCR8 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

Applications for DGCR8 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DGCR8

DGCR8 is a component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-mi

Alternate Names

C22orf12, DGCRK6chromosome 22 open reading frame 12, DiGeorge syndrome critical region 8, DiGeorge syndrome critical region gene 8, Gy1, microprocessor complex subunit DGCR8, pasha

Gene Symbol

DGCR8

Additional DGCR8 Products

Product Documents for DGCR8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DGCR8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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