DGCR8 [p Ser377] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29530

Novus Biologicals, part of Bio-Techne
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NBP2-29530
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DGCR8 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 conjugated to KLH. Accession # Q8WYQ5

Modification

p Ser377

Specificity

Specific for endogenous levels of the ~120 kDa DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGCR8 [p Ser377] Antibody

Western Blot: DGCR8 [p Ser377] Antibody [NBP2-29530]

Western Blot: DGCR8 [p Ser377] Antibody [NBP2-29530]

Western Blot: DGCR8 [p Ser377] Antibody [NBP2-29530] - Western blot of mouse nuclei lysate showing specific immunolabeling of the ~120k DGCR8 protein phosphorylated at Ser377 (control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).

Applications for DGCR8 [p Ser377] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DGCR8

The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010).

Alternate Names

C22orf12, DGCRK6chromosome 22 open reading frame 12, DiGeorge syndrome critical region 8, DiGeorge syndrome critical region gene 8, Gy1, microprocessor complex subunit DGCR8, pasha

Entrez Gene IDs

1644 (Human)

Gene Symbol

DDC

UniProt

Q8WYQ5 (Human)

Additional DGCR8 Products

Product Documents for DGCR8 [p Ser377] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DGCR8 [p Ser377] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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