DGK-epsilon Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85315
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for DGK-epsilon Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DGK-epsilon Antibody
![Immunocytochemistry/ Immunofluorescence: DGK-epsilon Antibody [NBP1-85315]](https://resources.bio-techne.com/images/products/DGK-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85315-img0004.jpg "Immunocytochemistry/ Immunofluorescence: DGK-epsilon Antibody [NBP1-85315]")
Immunocytochemistry/ Immunofluorescence: DGK-epsilon Antibody [NBP1-85315]
Immunocytochemistry/Immunofluorescence: DGK-epsilon Antibody [NBP1-85315] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm.![Immunohistochemistry-Paraffin: DGK-epsilon Antibody [NBP1-85315]](https://resources.bio-techne.com/images/products/DGK-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-85315-img0002.jpg "Immunohistochemistry-Paraffin: DGK-epsilon Antibody [NBP1-85315]")
Immunohistochemistry-Paraffin: DGK-epsilon Antibody [NBP1-85315]
Immunohistochemistry-Paraffin: DGK-epsilon Antibody [NBP1-85315] - Staining of human testis shows distinct positivity in spermatids and spermatozoa.Applications for DGK-epsilon Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DGK-epsilon
Long Name
Diacylglycerol Kinase, epsilon 64kDa
Alternate Names
DAGK5, DAGK6, DGKE, DGKepsilon
Gene Symbol
DGKE
Additional DGK-epsilon Products
Product Documents for DGK-epsilon Antibody
Product Specific Notices for DGK-epsilon Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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