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DGK-gamma Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33055

Novus Biologicals, part of Bio-Techne
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NBP1-33055

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DGK-gamma. The exact sequence is proprietary.

Localization

Cytoplasm, Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGK-gamma Antibody

Western Blot: DGK-gamma Antibody [NBP1-33055]

Western Blot: DGK-gamma Antibody [NBP1-33055]

Western Blot: DGK-gamma Antibody [NBP1-33055] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: DGK-gamma Antibody [NBP1-33055]

Immunocytochemistry/ Immunofluorescence: DGK-gamma Antibody [NBP1-33055]

Immunocytochemistry/Immunofluorescence: DGK-gamma Antibody [NBP1-33055] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: DGK-gamma Antibody [NBP1-33055]

Immunohistochemistry-Paraffin: DGK-gamma Antibody [NBP1-33055]

Immunohistochemistry-Paraffin: DGK-gamma Antibody [NBP1-33055] - NCIN87 xenograft, using DAG kinase gamma antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for DGK-gamma Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DGK-gamma

This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene.

Long Name

Diacylglycerol Kinase, gamma 90kDa

Alternate Names

DAGK3, DGKG, DGKgamma

Gene Symbol

DGKG

Additional DGK-gamma Products

Product Documents for DGK-gamma Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DGK-gamma Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.