DHCR7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80740

Novus Biologicals, part of Bio-Techne
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NBP1-80740
NBP1-80740-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DHCR7 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHCR7 Antibody

Immunocytochemistry/ Immunofluorescence: DHCR7 Antibody [NBP1-80740]

Immunocytochemistry/ Immunofluorescence: DHCR7 Antibody [NBP1-80740]

Immunocytochemistry/Immunofluorescence: DHCR7 Antibody [NBP1-80740] - Immunofluorescent staining of human cell line CACO-2 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP1-80740]

Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP1-80740]

Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP1-80740] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for DHCR7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHCR7

The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM]

Alternate Names

7-dehydrocholesterol reductase, 7-DHC reductase, D7SR, delta-7-dehydrocholesterol reductase, EC 1.3.1, EC 1.3.1.21, Putative sterol reductase SR-2, SLOS, Smith-Lemli-Opitz syndrome, Sterol Delta(7)-reductase, sterol delta-7-reductase

Entrez Gene IDs

1717 (Human)

Gene Symbol

DHCR7

Additional DHCR7 Products

Product Documents for DHCR7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DHCR7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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