DHCR7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12940

Novus Biologicals, part of Bio-Techne
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NBP3-12940
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DHCR7 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DHCR7. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for DHCR7 Antibody

Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940]

Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940]

Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940] - Rat duodenum. DHCR7 antibody (NBP3-12940) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940]

Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940]

Immunohistochemistry-Paraffin: DHCR7 Antibody [NBP3-12940] - Mouse liver. DHCR7 antibody (NBP3-12940) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
DHCR7 Antibody

Western Blot: DHCR7 Antibody [NBP3-12940] -

Western Blot: DHCR7 Antibody [NBP3-12940] -MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for DHCR7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
WB, As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHCR7

The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM]

Alternate Names

7-dehydrocholesterol reductase, 7-DHC reductase, D7SR, delta-7-dehydrocholesterol reductase, EC 1.3.1, EC 1.3.1.21, Putative sterol reductase SR-2, SLOS, Smith-Lemli-Opitz syndrome, Sterol Delta(7)-reductase, sterol delta-7-reductase

Gene Symbol

DHCR7

Additional DHCR7 Products

Product Documents for DHCR7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DHCR7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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