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DHX38 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40583

Novus Biologicals, part of Bio-Techne
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NB110-40583

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1200 and the C-terminus (residue 1227) of human DEAH (Asp-Glu-Ala-His) Box Protein 38 using the numbering given in entry NP_054722.2 (GeneID 9785).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DHX38 Antibody

Western Blot: DHX38 Antibody [NB110-40583]

Western Blot: DHX38 Antibody [NB110-40583]

Western Blot: DHX38 Antibody [NB110-40583] - Detection of Human and Mouse DHX38 on HeLa whole cell lystate using NB110-40583. DHX38 was also immunoprecipitated by rabbit anti-DHX38 antibody NB110-40582.
Immunohistochemistry-Paraffin: DHX38 Antibody [NB110-40583]

Immunohistochemistry-Paraffin: DHX38 Antibody [NB110-40583]

Immunohistochemistry-Paraffin: DHX38 Antibody [NB110-40583] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DHX38 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

Applications for DHX38 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHX38

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process.

Alternate Names

ATP-dependent RNA helicase DHX38, DDX38pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38, DEAH (Asp-Glu-Ala-His) box polypeptide 38, DEAH box protein 38, EC 3.6.1, EC 3.6.4.13, KIAA0224pre-mRNA splicing factor ATP-dependent RNA helicase PRP16, PRP16 homolog of S.cerevisiae, PRP16hPrp16, PRPF16

Entrez Gene IDs

9785 (Human); 64340 (Mouse)

Gene Symbol

DHX38

UniProt

Additional DHX38 Products

Product Documents for DHX38 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DHX38 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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