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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V78P3C10*D3

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Peptide Sequence - RHNGAISSK

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The clone number is listed as V78P3C10*D3 in the scientific literature.

Scientific Data Images for DIAPH-2 Antibody (V78P3C10*D3) - BSA Free

Western Blot: DIAPH-2 Antibody (V78P3C10*D3)BSA Free [NBP2-50229]

Western Blot: DIAPH-2 Antibody (V78P3C10*D3)BSA Free [NBP2-50229]

Western Blot: DIAPH-2 Antibody (V78P3C10-D3) [NBP2-50229] - Analysis of DIAPH-2 in Jurkat cell lysates.
Immunohistochemistry: DIAPH-2 Antibody (V78P3C10*D3) - BSA Free [NBP2-50229]

Immunohistochemistry: DIAPH-2 Antibody (V78P3C10*D3) - BSA Free [NBP2-50229]

Immunohistochemistry: DIAPH-2 Antibody (V78P3C10-D3) [NBP2-50229] - Analysis of DIAPH-2 in human colorectal cancer tissue. Showing cytoplasmic staining.

Applications for DIAPH-2 Antibody (V78P3C10*D3) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Recommend usage conditions for IHC: Neat, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. Positive control(s): ELISA - peptide immunogen; WB - Jurkat cell lysates; IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIAPH-2

DIAPH2 may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Long Name

Diaphanous Homolog 2

Alternate Names

DIA2, DIAPH2, DRF2, POF2

Gene Symbol

DIAPH2

Additional DIAPH-2 Products

Product Documents for DIAPH-2 Antibody (V78P3C10*D3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DIAPH-2 Antibody (V78P3C10*D3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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