DIAPH-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-280
![Western Blot: DIAPH-2 Antibody [NB600-280]](https://resources.bio-techne.com/images/products/DIAPH-2-Antibody-Western-Blot-NB600-280-img0004.jpg "Western Blot: DIAPH-2 Antibody [NB600-280]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Diaphanous Homolog 2 using the numbering given in entry NP_006720.1 (GeneID 1730).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
125.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DIAPH-2 Antibody
Western Blot: DIAPH-2 Antibody [NB600-280]
Western Blot: DIAPH-2 Antibody [NB600-280] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Diaphanous 2 antibody.![Western Blot: DIAPH-2 Antibody [NB600-280]](https://resources.bio-techne.com/images/products/DIAPH-2-Antibody-Western-Blot-NB600-280-img0002.jpg "Western Blot: DIAPH-2 Antibody [NB600-280]")
Western Blot: DIAPH-2 Antibody [NB600-280]
Western Blot: DIAPH-2 Antibody [NB600-280] - Whole cell lysate (50 ug) from HEK 293T cells. Antibody used at the indicated concentrations.Applications for DIAPH-2 Antibody
Application
Recommended Usage
Western Blot
1:1000-1:10000
Application Notes
This antibody can be used for Western blot. *Suggested Working Dilutions: Western blot: 1:1,000-1:10,000 Immunoprecipitation: not tested Immunohistochemistry: not tested *Optimal working dilutions should be determined experimentally by the investigator.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DIAPH-2
Long Name
Diaphanous Homolog 2
Alternate Names
DIA2, DIAPH2, DRF2, POF2
Entrez Gene IDs
1730 (Human)
Gene Symbol
DIAPH2
UniProt
Additional DIAPH-2 Products
Product Documents for DIAPH-2 Antibody
Product Specific Notices for DIAPH-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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