Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DIAPH1 Antibodies »

Product Specifications

Immunogen

Maps to a region between residues 1 and 50 of human Diaphanous Homolog 1 using the numbering given in entry NP_005210.1 (GeneID 1729).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DIAPH1 Antibody

Western Blot: DIAPH1 Antibody [NB600-230]

Western Blot: DIAPH1 Antibody [NB600-230]

Western Blot: DIAPH1 Antibody [NB600-230] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Diaphanous 1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).
Western Blot: DIAPH1 Antibody [NB600-230]

Western Blot: DIAPH1 Antibody [NB600-230]

Western Blot: DIAPH1 Antibody [NB600-230] - Whole cell lysate (10 or 50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 and 0.04 ug/ml.

Applications for DIAPH1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:10000-1:30000
Application Notes
By Western Blot, this antibody recognizes a band at ~205 kDa, representing Diaphanous 1. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DIAPH1

Diaphanous 1 is a member of the Formin homology (FH) family of cytoskeletal organizer proteins. It is required for cytokinesis and transcriptional activation of the serum response factor. It has also been suggested to contribute to localized actin polymerization and stress fiber formation, both by recruiting profiling to sites of Rho action and by cooperating with ROCK. The human Diaphanous 1 gene is associated with Premature Ovarian Failure (POF) and Nonsyndromic Deafness (DFNA1), an autosomal dominant, fully penetrant progressive hearing loss.

Alternate Names

DIA1, diaphanous homolog 1 (Drosophila), hDIA1, homolog) 1, LFHL1FLJ25265

Entrez Gene IDs

1729 (Human)

Gene Symbol

DIAPH1

Additional DIAPH1 Products

Product Documents for DIAPH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DIAPH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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