Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DIAPH1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1200 and the C-terminus (residue 1248) of human Diaphanous Homolog 1 using the numbering given in entry NP_005210.1 (GeneID 1729).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DIAPH1 Antibody

Western Blot: DIAPH1 Antibody [NB600-231]

Western Blot: DIAPH1 Antibody [NB600-231]

Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Immunohistochemistry: DIAPH1 Antibody [NB600-231]

Immunohistochemistry: DIAPH1 Antibody [NB600-231]

Immunohistochemistry: DIAPH1 Antibody [NB600-231] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Diaphanous used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB
Western Blot: DIAPH1 Antibody [NB600-231]

Western Blot: DIAPH1 Antibody [NB600-231]

Western Blot: DIAPH1 Antibody [NB600-231] - Whole cell lysate (50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 ug/ml.
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Applications for DIAPH1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Western Blot

1:5000-1:15000
Application Notes
By Western Blot this antibody recognizes a band at ~205 kDa, representing Diaphanous 1. Epitope Retrieval is not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DIAPH1

Diaphanous 1 is a member of the Formin homology (FH) family of cytoskeletal organizer proteins. It is required for cytokinesis and transcriptional activation of the serum response factor. It has also been suggested to contribute to localized actin polymerization and stress fiber formation, both by recruiting profiling to sites of Rho action and by cooperating with ROCK. The human Diaphanous 1 gene is associated with Premature Ovarian Failure (POF) and Nonsyndromic Deafness (DFNA1), an autosomal dominant, fully penetrant progressive hearing loss.

Alternate Names

DIA1, diaphanous homolog 1 (Drosophila), hDIA1, homolog) 1, LFHL1FLJ25265

Entrez Gene IDs

1729 (Human)

Gene Symbol

DIAPH1

UniProt

O60610 (Human)

Additional DIAPH1 Products

Product Documents for DIAPH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DIAPH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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