DIAPH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47202

Novus Biologicals, part of Bio-Techne
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NBP1-47202-0.1mg
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DIAPH1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QTKTSKAKKDQ corresponding to internal region according to NP_005210.3, NP_001073280.1.

Epitope

internal region

Specificity

This antibody is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DIAPH1 Antibody

Western Blot: DIAPH1 Antibody [NBP1-47202]

Western Blot: DIAPH1 Antibody [NBP1-47202]

Western Blot: DIAPH1 Antibody [NBP1-47202] - Analysis of NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-47202 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DIAPH1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 140 kDa band observed in NIH3T3 lysates (calculated MW of 141 kDa band according to human NP_005210.3 and 139 kDa band according to mouse NP_031884.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DIAPH1

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

DIA1, diaphanous homolog 1 (Drosophila), hDIA1, homolog) 1, LFHL1FLJ25265

Entrez Gene IDs

1729 (Human); 13367 (Mouse)

Gene Symbol

DIAPH1

UniProt

NP_005210.3 (Human)

Additional DIAPH1 Products

Product Documents for DIAPH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DIAPH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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