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DIAPH3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12956

Novus Biologicals, part of Bio-Techne
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NBP3-12956

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DIAPH3. The exact sequence is proprietary.

Reactivity Notes

Mouse (87%), Rat (87%), Bovine (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for DIAPH3 Antibody

Western Blot: DIAPH3 Antibody [NBP3-12956]

Western Blot: DIAPH3 Antibody [NBP3-12956]

Western Blot: DIAPH3 Antibody [NBP3-12956] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DIAPH3 antibody (NBP3-12956) diluted at 1:500.
Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP3-12956]

Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP3-12956]

Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP3-12956] - DIAPH3 antibody detects DIAPH3 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. DIAPH3 antibody (NBP3-12956) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for DIAPH3 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIAPH3

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to themembrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, andtranscriptional activation of the serum r

Alternate Names

DIAP3, diaphanous homolog 3 (Drosophila), diaphanous-related formin 3, DKFZp434C0931, DKFZp686A13178, homolog) 3, mDia2, protein diaphanous homolog 3

Gene Symbol

DIAPH3

Additional DIAPH3 Products

Product Documents for DIAPH3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DIAPH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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