Dicer Antibody (CL0378)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30699

Novus Biologicals, part of Bio-Techne
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NBP2-30699
NBP2-30699-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Virus

Cited:

Virus

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0378

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dicer Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLH

Epitope

LSSAEKRKAK

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions Use in Virus reported in scientific literature (PMID:32763144).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Dicer Antibody (CL0378)

Western Blot: Dicer Antibody (CL0378) [NBP2-30699]

Western Blot: Dicer Antibody (CL0378) [NBP2-30699]

Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Analysis of extracts from RT-4 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-DICER monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunocytochemistry/ Immunofluorescence: Dicer Antibody (CL0378) [NBP2-30699]

Immunocytochemistry/ Immunofluorescence: Dicer Antibody (CL0378) [NBP2-30699]

Immunocytochemistry/Immunofluorescence: Dicer Antibody (CL0378) [NBP2-30699] - Staining of A-431 cells using the anti-DICER1 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699]

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699]

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human breast cancer (ductal carcinoma) shows moderate cytoplasmic positivity in cancer cells.
View 7 more images

Applications for Dicer Antibody (CL0378)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dicer

Dicer encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Alternate Names

DCR1, Dicer, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, Dicer1, Dcr-1 homolog (Drosophila), EC 3.1.26, EC 3.1.26.-, EC 3.1.26.3, Helicase MOI, Helicase with RNase motif, helicase-moi, HERNADicer1, Dcr-1 homolog, K12H4.8-LIKE, KIAA0928endoribonuclease Dicer

Gene Symbol

DICER1

UniProt

Q9UPY3 (Human)

Additional Dicer Products

Product Documents for Dicer Antibody (CL0378)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dicer Antibody (CL0378)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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