Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S167-7

Concentration

1 mg/ml

View all Dicer Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer

Localization

Cytoplasm

Specificity

Detects approx 215kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Dicer Antibody (S167-7)

Western Blot: Dicer Antibody (S167-7) [NBP2-22415]

Western Blot: Dicer Antibody (S167-7) [NBP2-22415]

Western Blot: Dicer Antibody (S167-7) [NBP2-22415] - Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.
Immunocytochemistry/ Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415]

Immunocytochemistry/ Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415]

Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (NBP2-22415). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (NBP2-22415) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Dicer Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415]

Immunocytochemistry/ Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415]

Immunocytochemistry/Immunofluorescence: Dicer Antibody (S167-7) [NBP2-22415] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Dicer Antibody (D) Composite.

Applications for Dicer Antibody (S167-7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of Dicer Antibody was sufficient for detection of Dicer in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dicer

Dicer encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Alternate Names

DCR1, Dicer, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, Dicer1, Dcr-1 homolog (Drosophila), EC 3.1.26, EC 3.1.26.-, EC 3.1.26.3, Helicase MOI, Helicase with RNase motif, helicase-moi, HERNADicer1, Dcr-1 homolog, K12H4.8-LIKE, KIAA0928endoribonuclease Dicer

Gene Symbol

DICER1

UniProt

Q8R418 (Mouse)

Additional Dicer Products

Product Documents for Dicer Antibody (S167-7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dicer Antibody (S167-7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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