Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Dicer Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1175 and 1225 of human double-stranded RNA-specific endoribonuclease; dicer1 using the numbering given in entry NP_085124.2 (GeneID 23405).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25351346).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dicer Antibody

Western Blot: Dicer Antibody [NBP1-06521]

Western Blot: Dicer Antibody [NBP1-06521]

Western Blot: Dicer Antibody [NBP1-06521] - Whole cell lysate from 293T and HeLa cells. Dicer was also immunoprecipitated by rabbit anti-Dicer antibodies NBP1-06520 and another Dicer antibody.

Applications for Dicer Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in ICC/IF and IHC-Frozen reported in scientific literature (PMID 25351346).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Dicer

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Alternate Names

DCR1, Dicer, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, Dicer1, Dcr-1 homolog (Drosophila), EC 3.1.26, EC 3.1.26.-, EC 3.1.26.3, Helicase MOI, Helicase with RNase motif, helicase-moi, HERNADicer1, Dcr-1 homolog, K12H4.8-LIKE, KIAA0928endoribonuclease Dicer

Entrez Gene IDs

23405 (Human)

Gene Symbol

DICER1

UniProt

NP_085124.2 (Human)

Additional Dicer Products

Product Documents for Dicer Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Dicer Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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