DLL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-628

Novus Biologicals, part of Bio-Techne
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NB600-628
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Human (96%), Rat (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DLL1 Antibodies »

Product Summary for DLL1 Antibody

Immunogen

This affinity purified DLL1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 150-175 of Mouse DLL1. (Uniprot: Q61483)

Reactivity Notes

Activity against DLL11 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken.

Specificity

The antibody detects DLL1 from mouse. Activity against Delta-1 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for DLL1 Antibody

Western Blot: DLL1 Antibody [NB600-628]

Western Blot: DLL1 Antibody [NB600-628]

Western Blot: DLL1 Antibody [NB600-628]
Immunohistochemistry: DLL1 Antibody [NB600-628]

Immunohistochemistry: DLL1 Antibody [NB600-628]

Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibodyTissue: Mouse Embryonic Kidney. Fixation: FFPE buffered formalin 10% conc.Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker (left, right), EDTA pH 9.5 Pressure Cooker (middle).Primary antibody: 2ug/ml 1.5 hour @ room temp.Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45" RT.
Immunohistochemistry: DLL1 Antibody [NB600-628]

Immunohistochemistry: DLL1 Antibody [NB600-628]

Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibody. Tissue: Mouse embryonic kidney.Fixation: formalin fixed paraffin embedded 10% conc.Antigen retrieval: pH 9.5. Primary antibody: 2ug/ml for 1.5 hour @ room Temp.Secondary antibody: Peroxidase secondary reagent for 45 min at RT.Localization: as shown.Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
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Applications for DLL1 Antibody

Application
Recommended Usage

ELISA

1:3000-1:12000

Immunohistochemistry

2ug/ml

Western Blot

1:100-1:1000
Application Notes
This product has been tested for use in ELISA against the immunizing peptide, western blot, and in IHC. Reactivity in other immunoassays is unknown.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: DLL1

Delta 1 is a mouse homolog of the Notch Delta ligand and acts as a ligand for Notch receptors. Delta 1 is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. Delta 1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Long Name

Delta-like 1

Alternate Names

Delta 1

Gene Symbol

DLL1

UniProt

Q61483

Additional DLL1 Products

Product Documents for DLL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DLL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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