DNA-PKcs Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-659

Novus Biologicals, part of Bio-Techne
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NB100-659
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DNA-PKcs Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 2100 and 2150 of human DNA-Dependent Protein Kinase, catalytic subunit using the numbering given in entry NP_008835.5 (GeneID 5591).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA-PKcs Antibody

Western Blot: DNA-PKcs Antibody [NB100-659]

Western Blot: DNA-PKcs Antibody [NB100-659]

Western Blot: DNA-PKcs Antibody [NB100-659] - Detection of Human DNA-PKcs on HeLa whole cell lysate using NB100-659. DNA-PKcs was also IPed using rabbit anti-DNA-PKcs antibodies NB100-657, NB100-658 and NB100-660.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NB100-659]

Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NB100-659]

Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NB100-659] - Human lung carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

Applications for DNA-PKcs Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA-PKcs

DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Alternate Names

DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7

Entrez Gene IDs

5591 (Human)

Gene Symbol

PRKDC

UniProt

NP_008835.5 (Human)

Additional DNA-PKcs Products

Product Documents for DNA-PKcs Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DNA-PKcs Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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