DNA-PKcs Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33995

Novus Biologicals, part of Bio-Techne
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NBP2-33995-25ul
NBP2-33995
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNA-PKcs Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ETLSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESYSFKDTSTGHKNKEFVARIK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA-PKcs Antibody

Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody [NBP2-33995]

Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody [NBP2-33995]

Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody [NBP2-33995] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995]

Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995]

Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995]

Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995]

Immunohistochemistry-Paraffin: DNA-PKcs Antibody [NBP2-33995] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.
View 2 more images

Applications for DNA-PKcs Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA-PKcs

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.(supplied by OMIM)

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Alternate Names

DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7

Gene Symbol

PRKDC

UniProt

P78527

Additional DNA-PKcs Products

Product Documents for DNA-PKcs Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DNA-PKcs Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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