DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07616

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-POLE3-2F10

Concentration

0.2 mg/ml

View all DNA Polymerase epsilon subunit 3 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human DNA Polymerase epsilon subunit 3 protein (Uniprot: Q9NRF9)

Reactivity Notes

Predicted to react in Mouse, Rat and Xenopus.

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

16.86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08571)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10)

Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616]

Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616]

Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616] - Formalin-fixed, paraffin-embedded human lymphoma stained with DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10).
Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616]

Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616]

Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616] - Flow Cytometric Analysis of PFA-fixed K562 cells. DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).
Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616]

Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616]

Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-07616] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10).

Applications for DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: DNA Polymerase epsilon subunit 3

DNA polymerase epsilon (POLE) is an enzyme that helps catalyze in the polymerization of deoxyribonucleotides into a DNA strand. The C-terminus of DNA polymerase E subunit complexes with subunits B and C, whereas the N-terminal domain contains an active polymerase domain.

DNA Pol E antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Alternate Names

Arsenic-transactivated protein, AsTP, CHARAC17arsenic transactivated protein, CHRAC-17, CHRAC17Ybl1, Chromatin accessibility complex 17 kDa protein, DNA polymerase epsilon p17 subunit, DNA polymerase epsilon subunit 3, DNA polymerase epsilon subunit p17, DNA polymerase II subunit 3, EC 2.7.7.7, histone fold protein CHRAC17, HuCHRAC17, p17, polymerase (DNA directed), epsilon 3 (p17 subunit), YBL1

Gene Symbol

POLE3

UniProt

Q9NRF9 (Human)

Additional DNA Polymerase epsilon subunit 3 Products

Product Documents for DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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