DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08571
![Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]](https://resources.bio-techne.com/images/products/DNA-Polymerase-epsilon-subunit-3-Antibody-PCRP-POLE3-2F10-Immunohistochemistry-Paraffin-NBP3-08571-img0002.jpg "Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PCRP-POLE3-2F10
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human DNA Polymerase epsilon subunit 3 protein (Uniprot: Q9NRF9)
Reactivity Notes
Predicted to react in Mouse, Rat and Xenopus.
Localization
Nucleus, Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
16.86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07616).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images
Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]
Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Formalin-fixed, paraffin-embedded human lymphoma stained with DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10).![Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]](https://resources.bio-techne.com/images/products/DNA-Polymerase-epsilon-subunit-3-Antibody-PCRP-POLE3-2F10-Flow-Cytometry-NBP3-08571-img0001.jpg "Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]")
Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]
Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Flow Cytometric Analysis of PFA-fixed K562 cells. DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).![Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]](https://resources.bio-techne.com/images/products/DNA-Polymerase-epsilon-subunit-3-Antibody-PCRP-POLE3-2F10-Protein-Array-NBP3-08571-img0003.jpg "Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]")
Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) - Azide and BSA Free [NBP3-08571]
Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10).Applications
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: DNA Polymerase epsilon subunit 3
DNA Pol E antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.
Alternate Names
Arsenic-transactivated protein, AsTP, CHARAC17arsenic transactivated protein, CHRAC-17, CHRAC17Ybl1, Chromatin accessibility complex 17 kDa protein, DNA polymerase epsilon p17 subunit, DNA polymerase epsilon subunit 3, DNA polymerase epsilon subunit p17, DNA polymerase II subunit 3, EC 2.7.7.7, histone fold protein CHRAC17, HuCHRAC17, p17, polymerase (DNA directed), epsilon 3 (p17 subunit), YBL1
Gene Symbol
POLE3
Additional DNA Polymerase epsilon subunit 3 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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