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DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92951

Novus Biologicals, part of Bio-Techne
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NBP2-92951-0.02ml
NBP2-92951-0.1ml

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human DNA Polymerase epsilon subunit 3 (NP_059139.3). MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Western Blot: DNA Polymerase epsilon subunit 3 AntibodyBSA Free [NBP2-92951]

Western Blot: DNA Polymerase epsilon subunit 3 AntibodyBSA Free [NBP2-92951]

Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NBP2-92951] - Western blot analysis of extracts from normal (control) and [KO Validated] DNA Polymerase epsilon subunit 3 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] DNA Polymerase epsilon subunit 3 Rabbit pAb antibody (NBP2-92951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: DNA Polymerase epsilon subunit 3 AntibodyBSA Free [NBP2-92951]

Western Blot: DNA Polymerase epsilon subunit 3 AntibodyBSA Free [NBP2-92951]

Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NBP2-92951] - Analysis of extracts of various cell lines, using DNA Polymerase epsilon subunit 3 . Exposure time: 90s.

Applications for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA Polymerase epsilon subunit 3

DNA polymerase epsilon (POLE) is an enzyme that helps catalyze in the polymerization of deoxyribonucleotides into a DNA strand. The C-terminus of DNA polymerase E subunit complexes with subunits B and C, whereas the N-terminal domain contains an active polymerase domain.

DNA Pol E antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Alternate Names

Arsenic-transactivated protein, AsTP, CHARAC17arsenic transactivated protein, CHRAC-17, CHRAC17Ybl1, Chromatin accessibility complex 17 kDa protein, DNA polymerase epsilon p17 subunit, DNA polymerase epsilon subunit 3, DNA polymerase epsilon subunit p17, DNA polymerase II subunit 3, EC 2.7.7.7, histone fold protein CHRAC17, HuCHRAC17, p17, polymerase (DNA directed), epsilon 3 (p17 subunit), YBL1

Gene Symbol

POLE3

Additional DNA Polymerase epsilon subunit 3 Products

Product Documents for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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