DNA Polymerase gamma Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33633

Novus Biologicals, part of Bio-Techne
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NBP1-33633
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNA Polymerase gamma Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Polymerase gamma Antibody

Western Blot: DNA Polymerase gamma Antibody [NBP1-33633]

Western Blot: DNA Polymerase gamma Antibody [NBP1-33633]

Western Blot: DNA Polymerase gamma Antibody [NBP1-33633] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase gamma antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-33633]

Immunocytochemistry/ Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-33633]

Immunocytochemistry/Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-33633] - analysis of methanol-fixed HeLa.
Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-33633]

Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-33633]

Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-33633] - Human skeletal muscle, using DNA polymerase gamma antibody(10 ug/ml). Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for DNA Polymerase gamma Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DNA Polymerase gamma

As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil-provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.

Long Name

DNA polymerase subunit gamma-1

Alternate Names

MDP1, POLG, PolG-alpha, POLG1, POLGA

Entrez Gene IDs

5428 (Human); 85472 (Rat)

Gene Symbol

POLG

UniProt

P54098 (Human)

Additional DNA Polymerase gamma Products

Product Documents for DNA Polymerase gamma Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA Polymerase gamma Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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