DNA Polymerase lambda Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1358
Key Product Details
Species Reactivity
Human
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-LGLPYREPAERDW corresponding to C-Terminus according to NP_037406.1.
Localization
Nuclear
Specificity
This product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core), but considering only the peptide that we used as immunogen, the two share the sequence YREP**R** at the C-terminus. Furthermore, the protein structures appear to be very similar in this region.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for DNA Polymerase lambda Antibody
Western Blot: DNA Polymerase lambda Antibody [NB100-1358]
Western Blot: DNA Polymerase lambda Antibody [NB100-1358] - Staining of A549 (A) and HepG2 (B) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for DNA Polymerase lambda Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.5-1 ug/ml
Application Notes
WB: Approx 60 kDa band observed in cell lysates of A549 and approx 60-70 kDa in cell lysates of HepG2 (calculated MW of 63.5k Da according to Human NP_037406.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DNA Polymerase lambda
Alternate Names
BETAN, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, DNA polymerase lambda, EC 2.7.7.7, EC 4.2.99.-, FLJ46002, Pol beta2, Pol Lambda, POLKAPPA, polymerase (DNA directed), lambda
Gene Symbol
POLL
UniProt
Additional DNA Polymerase lambda Products
Product Documents for DNA Polymerase lambda Antibody
Product Specific Notices for DNA Polymerase lambda Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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