DNA Polymerase lambda Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-81664
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human polymerase (DNA directed), lambda using the numbering given in entry NP_037406.1 (GeneID 27343).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DNA Polymerase lambda Antibody
![Western Blot: DNA Polymerase lambda Antibody [NB100-81664]](https://resources.bio-techne.com/images/products/DNA-Polymerase-lambda-Antibody-Western-Blot-NB100-81664-img0003.jpg "Western Blot: DNA Polymerase lambda Antibody [NB100-81664]")
Western Blot: DNA Polymerase lambda Antibody [NB100-81664]
Western Blot: DNA Polymerase lambda Antibody [NB100-81664] - Detection of Human Pol Lambda by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81664 used for WB at 0.1 ug/ml. Detection : Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81664]](https://resources.bio-techne.com/images/products/DNA-Polymerase-lambda-Antibody-Immunoprecipitation-NB100-81664-img0004.jpg "Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81664]")
Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81664]
Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81664] - Detection of human Polymerase lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81664 used for IP at 6 ug per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81665. For blotting immunoprecipitated Pol Lambda, NB100-81664 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for DNA Polymerase lambda Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DNA Polymerase lambda
Alternate Names
BETAN, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, DNA polymerase lambda, EC 2.7.7.7, EC 4.2.99.-, FLJ46002, Pol beta2, Pol Lambda, POLKAPPA, polymerase (DNA directed), lambda
Entrez Gene IDs
27343 (Human)
Gene Symbol
POLL
UniProt
Additional DNA Polymerase lambda Products
Product Documents for DNA Polymerase lambda Antibody
Product Specific Notices for DNA Polymerase lambda Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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