DNA Polymerase lambda Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81665

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DNA Polymerase lambda Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human polymerase (DNA directed), lambda using the numbering given in entry NP_037406.1 (GeneID 27343).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Polymerase lambda Antibody

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665]

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665]

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665] - Detection of human Pol Lambda by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pol Lambda (NB100-81665). Detection: DAB
Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665]

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665]

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665] - Detection of mouse Pol Lambda by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Pol Lambda (NB100-81665). Detection: DAB
Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665]

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665]

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665] - Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81665 used for IP at 6 ug per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81664. For blotting immunoprecipitated Pol Lambda, NB100-81665 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DNA Polymerase lambda Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA Polymerase lambda

DNA polymerase lambda (pol lambda) is a DNA polymerase involved in base excision repair (BER). Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Alternate names for pol lambda include DNA polymerase kappa, DNA polymerase beta-2, pol beta-2, POLL, POLL-KAPPA, and BETA-N.

Alternate Names

BETAN, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, DNA polymerase lambda, EC 2.7.7.7, EC 4.2.99.-, FLJ46002, Pol beta2, Pol Lambda, POLKAPPA, polymerase (DNA directed), lambda

Entrez Gene IDs

27343 (Human)

Gene Symbol

POLL

UniProt

NP_037406.1 (Human)

Additional DNA Polymerase lambda Products

Product Documents for DNA Polymerase lambda Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA Polymerase lambda Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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