DNMT1 Antibody (11H8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59694

Novus Biologicals, part of Bio-Techne
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NBP2-59694
NBP2-59694-25ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 11H8

Concentration

1 mg/ml

View all DNMT1 Antibodies »

Product Summary for DNMT1 Antibody (11H8)

Immunogen

Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

Localization

Nucleus

Specificity

It will cross-react with mouse DNMT1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNMT1 Antibody (11H8)

Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (11H8) [NBP2-59694]

Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (11H8) [NBP2-59694]

Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (11H8) [NBP2-59694] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 11H8 (NBP2-59694). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (NBP2-59694) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) DNMT1 Antibody. (D) Composite.

Applications for DNMT1 Antibody (11H8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
2 ug/ml of this antibody was sufficient for detection of Dnmt1 in 10 ug of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 50% Glycerol, 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNMT1

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

Long Name

DNA [Cytosine-5-]-Methyltransferase 1

Alternate Names

CXXC9, MCMT

Gene Symbol

DNMT1

Additional DNMT1 Products

Product Documents for DNMT1 Antibody (11H8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DNMT1 Antibody (11H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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