DNMT1 Antibody (A3A8-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32274
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A3A8-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Dnmt1 aa 500-600 / 1616. (Uniprot: P26358)
Localization
Nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
183 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DNMT1 Antibody (A3A8-R)
Western Blot: DNMT1 Antibody (A3A8-R) [NBP3-32274]
Western Blot: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/1,000 dilution.Lane 1: HeLa cell lysate, 20 ug/Lane
Lane 2: 293T cell lysate, 20 ug/Lane
Lane 3: HepG2 cell lysate, 20 ug/Lane
Lane 4: Jurkat cell lysate, 20 ug/Lane
Predicted band size: 183 kDa
Observed band size: 183 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (A3A8-R) [NBP3-32274]
Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Immunocytochemistry analysis of HeLa cells labeling DNMT1 with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.
Applications for DNMT1 Antibody (A3A8-R)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNMT1
Improper methylation patterns have been linked to certain human tumors and developmental abnormalities. DNMT1 antibodies are useful tools for DNA methlyation research and studies on certian cancer.
Long Name
DNA [Cytosine-5-]-Methyltransferase 1
Alternate Names
CXXC9, MCMT
Gene Symbol
DNMT1
Additional DNMT1 Products
Product Documents for DNMT1 Antibody (A3A8-R)
Product Specific Notices for DNMT1 Antibody (A3A8-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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