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DNTTIP1 Antibody (OTI1F4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02507

Novus Biologicals, part of Bio-Techne
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NBP2-02507

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F4

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human DNTTIP1 (NP_443183) produced in HEK293T cell.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32297854). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNTTIP1 Antibody (OTI1F4)

Western Blot: DNTTIP1 Antibody (OTI1F4) [NBP2-02507]

Western Blot: DNTTIP1 Antibody (OTI1F4) [NBP2-02507]

Western Blot: DNTTIP1 Antibody (OTI1F4) [NBP2-02507] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNTTIP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNTTIP1.
Immunocytochemistry/ Immunofluorescence: DNTTIP1 Antibody (OTI1F4) [NBP2-02507]

Immunocytochemistry/ Immunofluorescence: DNTTIP1 Antibody (OTI1F4) [NBP2-02507]

Immunocytochemistry/Immunofluorescence: DNTTIP1 Antibody (OTI1F4) [NBP2-02507] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DNTTIP1.
Immunohistochemistry-Paraffin: DNTTIP1 Antibody (OTI1F4) [NBP2-02507]

Immunohistochemistry-Paraffin: DNTTIP1 Antibody (OTI1F4) [NBP2-02507]

Immunohistochemistry-Paraffin: DNTTIP1 Antibody (OTI1F4) [NBP2-02507] - Staining of paraffin-embedded prostate tissue using anti-DNTTIP1 mouse monoclonal antibody.

Applications for DNTTIP1 Antibody (OTI1F4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNTTIP1

DNTTIP1 binds DNA and enhances the activity of terminal deoxynucleotidyltransferase (TDT, or DNTT; MIM 187410), a DNA polymerase that catalyzes the polymerization of DNA in the absence of a DNA template

Alternate Names

C20orf167, chromosome 20 open reading frame 167, deoxynucleotidyltransferase terminal-interacting protein 1, deoxynucleotidyltransferase, terminal, interacting protein 1, dJ447F3.4, TdIF1, TdT binding protein, TdT-interacting factor 1, Terminal deoxynucleotidyltransferase-interacting factor 1

Entrez Gene IDs

116092 (Human)

Gene Symbol

DNTTIP1

Additional DNTTIP1 Products

Product Documents for DNTTIP1 Antibody (OTI1F4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNTTIP1 Antibody (OTI1F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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