DOCK10 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-60669
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human dedicator of cytokinesis 10 using the numbering given in entry NP_055504.2 (GeneID 55619).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25729399)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
249.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DOCK10 Antibody
Western Blot: DOCK10 Antibody [NB100-60669]
Western Blot: DOCK10 Antibody [NB100-60669] - Whole cell lysate (50 ug) from Jurkat, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK10 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Western Blot: DOCK10 Antibody [NB100-60669]
Western Blot: DOCK10 Antibody [NB100-60669] - Detection of Human DOCK10 on HeLa whole cell lystate using NB100-60670.Knockout Validated: DOCK10 Antibody [NB100-60669]
Knockout Validated: DOCK10 Antibody [NB100-60669] - Absence of Zizimin proteins, but normal numbers of hematopoietic cells in KO mice. Western blotting for Ziz2 (A) or Ziz3 (DOCK10) (B) Protein lysates (30 ug/lane) were loaded and designated antibodies were reacted with the membranes. Replicated membranes were used for all antibodies. Zizimin proteins were absent in KO mice (8 weeks old, male). Image collected and cropped by CiteAb from the following publication (https://www.immunityageing.com/content/12/1/1), licensed under a CC-BY license.Applications for DOCK10 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DOCK10
Alternate Names
dedicator of cytokinesis 10, dedicator of cytokinesis protein 10, DKFZp781A1532, DRIP2, KIAA0694protein zizimin 3, Nbla10300, ZIZ3dopamine receptor interacting protein 2, zizimin3, Zizimin-3
Entrez Gene IDs
55619 (Human)
Gene Symbol
DOCK10
UniProt
Additional DOCK10 Products
Product Documents for DOCK10 Antibody
Product Specific Notices for DOCK10 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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