Skip to main content

DOCK10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60669

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human dedicator of cytokinesis 10 using the numbering given in entry NP_055504.2 (GeneID 55619).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25729399)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

249.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DOCK10 Antibody

Western Blot: DOCK10 Antibody [NB100-60669]

Western Blot: DOCK10 Antibody [NB100-60669]

Western Blot: DOCK10 Antibody [NB100-60669] - Whole cell lysate (50 ug) from Jurkat, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK10 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: DOCK10 Antibody [NB100-60669]

Western Blot: DOCK10 Antibody [NB100-60669]

Western Blot: DOCK10 Antibody [NB100-60669] - Detection of Human DOCK10 on HeLa whole cell lystate using NB100-60670.
Knockout Validated: DOCK10 Antibody [NB100-60669]

Knockout Validated: DOCK10 Antibody [NB100-60669]

Knockout Validated: DOCK10 Antibody [NB100-60669] - Absence of Zizimin proteins, but normal numbers of hematopoietic cells in KO mice. Western blotting for Ziz2 (A) or Ziz3 (DOCK10) (B) Protein lysates (30 ug/lane) were loaded and designated antibodies were reacted with the membranes. Replicated membranes were used for all antibodies. Zizimin proteins were absent in KO mice (8 weeks old, male). Image collected and cropped by CiteAb from the following publication (https://www.immunityageing.com/content/12/1/1), licensed under a CC-BY license.

Applications for DOCK10 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DOCK10

DOCK10 is a potential Rho-guanine nucleotide exchange factor (Rho-GEF) that may function to activate small GTPases such as Rac and Cdc42. It is a member of the CZH (CDM-zizimin homology) family of proteins that contain two highly conserved domains, CZH1 and CZH2.

Alternate Names

dedicator of cytokinesis 10, dedicator of cytokinesis protein 10, DKFZp781A1532, DRIP2, KIAA0694protein zizimin 3, Nbla10300, ZIZ3dopamine receptor interacting protein 2, zizimin3, Zizimin-3

Entrez Gene IDs

55619 (Human)

Gene Symbol

DOCK10

UniProt

Additional DOCK10 Products

Product Documents for DOCK10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DOCK10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...