Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DG1/447

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ANO1/TMEM16A Antibodies »

Product Specifications

Immunogen

Recombinant human canine1/TMEM16A protein (Uniprot: Q5XX6)

Localization

Cell Surface and Cytoplasmic

Specificity

Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%.

Marker

Gastrointestinal Stromal Tumor Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for DOG1/TMEM16A Antibody (DG1/447) - IHC-Prediluted

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/447) - IHC-Prediluted [NBP2-48152]

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/447) - IHC-Prediluted [NBP2-48152]

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/447) - IHC-Prediluted [NBP2-48152] - Human GIST stained with DOG-1 Mouse Monoclonal Antibody (DG1/447).

Applications for DOG1/TMEM16A Antibody (DG1/447) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ANO1/TMEM16A

Long Name

Anoctamin 1

Alternate Names

DOG1, ORAOV2, TAOS2, TMEM16A

Gene Symbol

ANO1

Additional ANO1/TMEM16A Products

Product Documents for DOG1/TMEM16A Antibody (DG1/447) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DOG1/TMEM16A Antibody (DG1/447) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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