DOG1/TMEM16A Antibody (PO00-27)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33029
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PO00-27
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human DOG1/TMEM16A aa 400-500. (Uniprot: Q5XXA6)
Localization
Cytoplasm, Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DOG1/TMEM16A Antibody (PO00-27)
Immunohistochemistry: DOG1/TMEM16A Antibody (PO00-27) [NBP3-33029]
Immunohistochemistry: DOG1/TMEM16A Antibody (PO00-27) [NBP3-33029] - Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue with Rabbit anti-DOG1/TMEM16A antibody (NBP3-33029) at 1/1,500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33029) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for DOG1/TMEM16A Antibody (PO00-27)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000-1:1500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ANO1/TMEM16A
Long Name
Anoctamin 1
Alternate Names
DOG1, ORAOV2, TAOS2, TMEM16A
Gene Symbol
ANO1
Additional ANO1/TMEM16A Products
Product Documents for DOG1/TMEM16A Antibody (PO00-27)
Product Specific Notices for DOG1/TMEM16A Antibody (PO00-27)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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