DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34812
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM580
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human canine1/TMEM16A protein (Uniprot: Q5XX6)
Localization
Cell Surface and Cytoplasm
Marker
Marker for Gastrointestinal Stromal Tumors
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32841).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free [NBP2-34812]
Immunocytochemistry/Immunofluorescence: DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free [NBP2-34812] - HEK cells expressing Ano1-Myc. SPM580 gave more signal than the Myc Ab control and much more than the SP31 mAb. Negative control cells were negative. ICC image submitted by a verified customer review.Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free [NBP2-34812]
Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free [NBP2-34812] - Formalin-fixed paraffin-embedded human GIST stained with DOG1 Monoclonal Antibody (SPM580).Applications for DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Validated for ICC/IF & WB from a verified customer review.
Optimal dilution for a specific application should be determined.
Validated for ICC/IF & WB from a verified customer review.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ANO1/TMEM16A
Long Name
Anoctamin 1
Alternate Names
DOG1, ORAOV2, TAOS2, TMEM16A
Gene Symbol
ANO1
Additional ANO1/TMEM16A Products
Product Documents for DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free
Product Specific Notices for DOG1/TMEM16A Antibody (SPM580) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...