DOK1 [p Tyr398] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82113

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-82113
NB100-82113-0.025ml
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DOK1 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human DOK1 around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).

Modification

p Tyr398

Specificity

Detects endogenous levels of DOK1 only when phosphorylated at tyrosine 398.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DOK1 [p Tyr398] Antibody

Western Blot: DOK1 [p Tyr398] Antibody [NB100-82113]

Western Blot: DOK1 [p Tyr398] Antibody [NB100-82113]

Western Blot: DOK1 [p Tyr398] Antibody [NB100-82113] - Analysis of extracts from K562 cells untreated or treated with H2O2 using p62Dok(phospho-Tyr398) antibody

Immunohistochemistry-Paraffin: DOK1 [p Tyr398] Antibody [NB100-82113] -

Immunohistochemistry-Paraffin: DOK1 [p Tyr398] Antibody [NB100-82113] - Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using NB100-82113 (left) or the same antibody preincubated with blocking peptide(right).

Applications for DOK1 [p Tyr398] Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DOK1

DOK1 (P62dok, Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 is a major substrate for many tyrosine kinases including c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation by kinases, DOK1 forms a complex with ras GTPase-activating protein. Phosphorylation on tyrosine residues by the insulin receptor kinase results in the negative regulation of the insulin signaling pathway. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.

Alternate Names

docking protein 1, docking protein 1 (downstream of tyrosine kinase 1), docking protein 1, 62kD (downstream of tyrosine kinase 1), docking protein 1, 62kDa (downstream of tyrosine kinase 1), Downstream of tyrosine kinase 1, MGC117395, MGC138860, p62(dok), p62dok, pp62

Entrez Gene IDs

1796 (Human); 312477 (Rat)

Gene Symbol

DOK1

UniProt

Q99704 (Human)

Additional DOK1 Products

Product Documents for DOK1 [p Tyr398] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DOK1 [p Tyr398] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.