Dopa Decarboxylase/DDC Antibody (CL2962) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43802

Novus Biologicals, part of Bio-Techne
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NBP3-43802
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NBP2-46642
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2962

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Dopa Decarboxylase/DDC Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P20711, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Dopa Decarboxylase/DDC Antibody (CL2962) - Azide and BSA Free

Dopa Decarboxylase/DDC Antibody (CL2962) - Azide and BSA Free Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP3-43802]

Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP3-43802]

Analysis in human kidney and fallopian tube tissues using NBP3-43802 antibody. Corresponding Dopa Decarboxylase/DDC RNA-seq data are presented for the same tissues.
Dopa Decarboxylase/DDC Antibody (CL2962) - Azide and BSA Free Western Blot: Dopa Decarboxylase/DDC Antibody [NBP3-43802]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP3-43802]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Kidney tissue
Dopa Decarboxylase/DDC Antibody (CL2962) - Azide and BSA Free Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP3-43802]

Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP3-43802]

Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
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Applications for Dopa Decarboxylase/DDC Antibody (CL2962) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopa Decarboxylase/DDC

DOPA Decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Alternate Names

AADC, DDC

Gene Symbol

DDC

Additional Dopa Decarboxylase/DDC Products

Product Documents for Dopa Decarboxylase/DDC Antibody (CL2962) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dopa Decarboxylase/DDC Antibody (CL2962) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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