Dopa Decarboxylase/DDC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-174

Novus Biologicals, part of Bio-Techne
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NB300-174
NB300-174-0.02ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human, Mouse, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Dopa Decarboxylase/DDC Antibodies »

Product Specifications

Immunogen

SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies. Accession # P27718

Reactivity Notes

Porcine reactivity reported in (PMID: 19857468).

Specificity

Specific for endogenous levels of the ~55 kDa Dopa Decarboxylase/DDC protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dopa Decarboxylase/DDC Antibody

Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174]

Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174]

Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.
Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174]

Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174]

Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla showing specific immunolabeling of the ~55k DDC protein.
Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174]

Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174]

Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174] - Simple Western lane view shows a specific band for DOPA Decarboxylase in 0.2 mg/ml of Mouse Cerebellum lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

Applications for Dopa Decarboxylase/DDC Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Simple Western

1:500

Western Blot

1:1000
Application Notes
This antibody can be used in Western blot where a single band at 55 kDa is seen. Immunohistochemistry-Paraffin was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dopa Decarboxylase/DDC

DOPA decarboxylase (DDC) is responsible for the synthesis of the neurotransmitters dopamine and serotonin. These two neurotransmitters play key roles in many clinical disorders, including Parkinson's disease.

Alternate Names

AADC, DDC

Entrez Gene IDs

24311 (Rat); 280762 (Bovine)

Gene Symbol

DDC

UniProt

P27718 (Bovine)

Additional Dopa Decarboxylase/DDC Products

Product Documents for Dopa Decarboxylase/DDC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dopa Decarboxylase/DDC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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