Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25442
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1163
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Dopamine beta-Hydroxylase. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%), Rhesus Macaque (86%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] -
Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.
Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] -
Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] -
Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Dopamine beta-Hydroxylase
Alternate Names
DBH, DBM, Dopamine betaHydroxylase, DOPBHY
Gene Symbol
DBH
Additional Dopamine beta-Hydroxylase Products
Product Documents for Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free
Product Specific Notices for Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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