Dopamine beta-Hydroxylase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57836

Novus Biologicals, part of Bio-Techne
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NBP2-57836
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dopamine beta-Hydroxylase Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKSSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQ

Reactivity Notes

Mouse 80%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dopamine beta-Hydroxylase Antibody

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Analysis in human adrenal gland and testis tissues. Corresponding Dopamine beta-Hydroxylase RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836]

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human adrenal gland, kidney, lower gastrointestinal and testis using Anti-Dopamine beta-Hydroxylase antibody NBP2-57836 (A) shows similar protein distribution across tissues to independent antibody NBP2-55677 (B).
Immunocytochemistry/ Immunofluorescence: Dopamine beta-Hydroxylase Antibody [NBP2-57836]

Immunocytochemistry/ Immunofluorescence: Dopamine beta-Hydroxylase Antibody [NBP2-57836]

Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles. Antibody staining is shown in green.
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Applications for Dopamine beta-Hydroxylase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopamine beta-Hydroxylase

Dopamine beta-hydroxylase belongs to the copper type II, ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of dopamine to noradrenaline. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons being expressed in noradrenergic and adrenergic neurons. DBH serves as a marker of noradrenergic cells.

Alternate Names

DBH, DBM, Dopamine betaHydroxylase, DOPBHY

Gene Symbol

DBH

Additional Dopamine beta-Hydroxylase Products

Product Documents for Dopamine beta-Hydroxylase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dopamine beta-Hydroxylase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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