Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80700

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Rat, Bovine (Negative), Canine (Negative), Human (Negative), Porcine (Negative), Rabbit (Negative), Sheep (Negative)

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SG2-D1a

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Dopamine D1R/DRD1 Antibodies »

Product Specifications

Immunogen

Recombinant rat Dopamine Receptor D1 protein near the C-terminus. [Swiss-Prot# P18901]

Localization

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free

Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a)Azide and BSA Free [NBP2-80700]

Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a)Azide and BSA Free [NBP2-80700]

Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone detected D1a receptor only and was not cross-reactive against DR-D1b (Note: these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.) Image from the standard format of this antibody.
Immunocytochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700]

Immunocytochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700]

Immunocytochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from verified customer review. Image from the standard format of this antibody.
Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700]

Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700]

Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free [NBP2-80700] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine D1 R antibody. Image from verified customer review. Image from the standard format of this antibody.

Applications for Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:1000

Western Blot

1:200 - 1:500
Application Notes
In Western blot multiple bands are seen around 45 and 100 kDa (dimer) in SF9 cell lysates overexpressing D1a and a single band at 90 kDa in striatal tissue lysate. Do NOT boil samples.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dopamine D1R/DRD1

Dopamine is a unique brain neurotransmitter which is responsible for the regulation of versatile processes such as motor activity, cognition, motivation, emotion, food intake, endocrine secretions etc. and dysfunctions in dopaminergic systems have been linked to drug addiction, schizophrenia, Parkinson's disease, Tourette's syndrome, attention-deficit and hyperactivity disorders as well as hyperprolactinemia. Dopamine Receptor D1 (DRD1), a GPCR protein, is the D1 subtype of dopamine receptors (DR) which represents the most abundant DR in CNS with capability of stimulating adenylyl cyclase and activating cyclic AMP-dependent protein kinases. DRD1 localize to cell membrane/ER membrane as multi-pass membrane protein and its transport from ER to cell surface is regulated by interaction with DNAJC14. DRD1 is highly expressed in the brain tissues, especially in the striatum, the nucleus accumbens, and the olfactory tubercle. DRD1 regulate neuronal growth and development, mediate behavioral responses, and modulate dopamine receptor D2-mediated events.

Long Name

Dopamine D1 Receptor

Alternate Names

DADR, Dopamine D1 R, DRD1

Gene Symbol

DRD1

Additional Dopamine D1R/DRD1 Products

Product Documents for Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Dopamine D1R/DRD1 Antibody (SG2-D1a) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.