Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25443

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25443
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1478

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dopamine D2 R/DRD2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-term region of Human Dopamine D2R/DRD2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%), Canine (84%), Porcine (88%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:1000 and competitor's antibody (Ab5084p) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Unboiled mouse tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084p) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] -

Western Blot: Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free [NBP3-25443] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine Receptor D2 antibody [HL1478] (NBP3-25443) diluted at 1:500 and competitor's antibody (Ab5084P) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Applications for Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Application
Recommended Usage

Functional Assay

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopamine D2 R/DRD2

The DRD2 Dopamine Receptor is involved in the neuromodulation of appetitive behaviors. DRD2 may be linked to alcoholism and other substance-use disorders. Mutations in DRD2 in mice lead to the suppression of reward behavior with morphine and to the reduction of spontaneous movements, a phenotype resembling Parkinson's disease. Three alternatively spliced isoforms have been identified. DRD2 has been reported to be expressed in various regions of the brain, as well as in aorta, artery, eye, and spinal cord. ESTs have been isolated from normal pituitary and in brain, colon, lung, pancreas, and skeletal muscle cancer libraries.

Long Name

Dopamine D2 Receptor

Alternate Names

D2DR, D2R, Dopamine D2R, DRD2

Gene Symbol

DRD2

Additional Dopamine D2 R/DRD2 Products

Product Documents for Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dopamine D2R/DRD2 Antibody (HL1478) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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