DOT1L Antibody (OTI1D8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72297
![Western Blot: DOT1L Antibody (OTI1D8)Azide and BSA Free [NBP2-72297]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-OTI1D8-Azide-and-BSA-Free-Western-Blot-NBP2-72297-img0001.jpg "Western Blot: DOT1L Antibody (OTI1D8)Azide and BSA Free [NBP2-72297]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1D8
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-434 of human DOT1L (NP_115871) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for DOT1L Antibody (OTI1D8) - Azide and BSA Free
Western Blot: DOT1L Antibody (OTI1D8)Azide and BSA Free [NBP2-72297]
Western Blot: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L.![Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-OTI1D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72297-img0002.jpg "Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]")
Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]
Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-OTI1D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72297-img0003.jpg "Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]")
Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]
Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for DOT1L Antibody (OTI1D8) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DOT1L
Long Name
Disruptor of Telomeric Silencing 1-like
Alternate Names
H3-K79-HMTase, KMT4
Gene Symbol
DOT1L
Additional DOT1L Products
Product Documents for DOT1L Antibody (OTI1D8) - Azide and BSA Free
Product Specific Notices for DOT1L Antibody (OTI1D8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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