Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DOT1L Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-434 of human DOT1L (NP_115871) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for DOT1L Antibody (OTI1D8) - Azide and BSA Free

Western Blot: DOT1L Antibody (OTI1D8)Azide and BSA Free [NBP2-72297]

Western Blot: DOT1L Antibody (OTI1D8)Azide and BSA Free [NBP2-72297]

Western Blot: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L.
Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]

Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]

Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]

Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297]

Immunohistochemistry: DOT1L Antibody (OTI1D8) - Azide and BSA Free [NBP2-72297] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for DOT1L Antibody (OTI1D8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Gene Symbol

DOT1L

Additional DOT1L Products

Product Documents for DOT1L Antibody (OTI1D8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DOT1L Antibody (OTI1D8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.