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DOT1L Antibody (S01-7F6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15025

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-15025-100ul
NBP3-15025-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-7F6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for DOT1L Antibody (S01-7F6)

Immunogen

Recombinant protein of human DOT1L (Uniprot#: Q8TEK3)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DOT1L Antibody (S01-7F6)

Western Blot: DOT1L Antibody (S01-7F6) [NBP3-15025]

Western Blot: DOT1L Antibody (S01-7F6) [NBP3-15025]

Western Blot: DOT1L Antibody (S01-7F6) [NBP3-15025] - Western blot detection of DOT1L in Hela, A549, HL-60 cell lysates using DOT1L Rabbit mAb (1:1000 diluted). Predicted band size: 165kDa. Observed band size: 185kDa.

Applications for DOT1L Antibody (S01-7F6)

Application
Recommended Usage

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Gene Symbol

DOT1L

Additional DOT1L Products

Product Documents for DOT1L Antibody (S01-7F6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DOT1L Antibody (S01-7F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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