Doublecortin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69992

Novus Biologicals, part of Bio-Techne
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NBP2-69992
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Doublecortin/DCX Antibodies »

Product Specifications

Immunogen

Peptide with sequence DRSHCQSLRFHQN, from the internal region of the protein sequence according to NP_000546.2.

Reactivity Notes

Expected from sequence similarity: Cow

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Doublecortin Antibody

Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP2-69992]

Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP2-69992]

Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-69992] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Doublecortin Antibody [NBP2-69992]

Flow Cytometry: Doublecortin Antibody [NBP2-69992]

Flow Cytometry: Doublecortin Antibody [NBP2-69992] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992]

Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992]

Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992] - (6ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Applications for Doublecortin Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

6 ug/mL

Peptide ELISA

1:6000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

TBS (pH 7.3), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Doublecortin/DCX

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Long Name

Doublecortin

Alternate Names

DBCN, Doublecortex, Doublin, Lis-X, LISX

Gene Symbol

DCX

Additional Doublecortin/DCX Products

Product Documents for Doublecortin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Doublecortin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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