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DREF Antibody (5G1)

Novus Biologicals, part of Bio-Techne | Catalog # H00009189-M01

Novus Biologicals, part of Bio-Techne
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H00009189-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5G1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ZBED1 (NP_004720, 3 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMAQIAYSGNTSNLSYHLEKNHPEEFCEFVKSNTEQMREAFATAFSKLKP

Reactivity Notes

Human. Other species not tested.

Specificity

ZBED1 - zinc finger, BED-type containing 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for DREF Antibody (5G1)

Western Blot: DREF Antibody (5G1) [H00009189-M01]

Western Blot: DREF Antibody (5G1) [H00009189-M01]

Western Blot: DREF Antibody (5G1) [H00009189-M01] - ZBED1 monoclonal antibody (M01), clone 5G1 Analysis of ZBED1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: DREF Antibody (5G1) [H00009189-M01]

Immunocytochemistry/ Immunofluorescence: DREF Antibody (5G1) [H00009189-M01]

Immunocytochemistry/Immunofluorescence: DREF Antibody (5G1) [H00009189-M01] - Analysis of monoclonal antibody to ZBED1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: DREF Antibody (5G1) [H00009189-M01]

Western Blot: DREF Antibody (5G1) [H00009189-M01]

Western Blot: DREF Antibody (5G1) [H00009189-M01] - Analysis of ZBED1 expression in transfected 293T cell line by ZBED1 monoclonal antibody (M01), clone 5G1.Lane 1: ZBED1 transfected lysate(78.2 KDa).Lane 2: Non-transfected lysate.

Applications for DREF Antibody (5G1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DREF

DREF binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable el

Alternate Names

Ac-like transposable element, ALTEDNA replication-related element binding factor, hDREF, KIAA0785dREF homolog, Putative Ac-like transposable element, putative Ac-like transposon, TRAMPDREFBED-type zinc finger domain-containing protein 1, zinc finger BED domain-containing protein 1, zinc finger, BED-type containing 1

Entrez Gene IDs

9189 (Human)

Gene Symbol

ZBED1

OMIM

300178 (Human)

Additional DREF Products

Product Documents for DREF Antibody (5G1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DREF Antibody (5G1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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