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DRIL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38452

Novus Biologicals, part of Bio-Techne
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NBP3-38452-100ul
NBP3-38452-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human DRIL1 (NP_005215.1).

Sequence:
PYECEKRGLSNPNELQAAIDSNRREGRRQSFGGSLFAYSPGGAHGMLSSPKLPVSSLGLAASTNGSSITPAPKIKKEEDSAIPITVPGRLPVSLAGHPVVAAQAAAVQAAAAQAAVAAQAAALEQLREKLESAEPPEKKMALVADEQQRLMQRALQQNFLAMAAQLPMSIRINSQASESRQDSAVNLTGTNGSNSISMSVEINGIMYTGVLFAQPPAPTPTSAPNKGGGGGGGSSSNAGGRGGNTGTSGGQAGPAGLSTPSTSTSNNSLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DRIL1 Antibody

DRIL1 Antibody

Immunohistochemistry: DRIL1 Antibody [NBP3-38452] -

Immunohistochemistry: DRIL1 Antibody [NBP3-38452] - Immunohistochemistry analysis of paraffin-embedded Rat lung using DRIL1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
DRIL1 Antibody

Immunoprecipitation: DRIL1 Antibody [NBP3-38452] -

Immunoprecipitation: DRIL1 Antibody [NBP3-38452] - Immunoprecipitation analysis of 150 ug extracts of MCF7 cells using 3 ug DRIL1 antibody. Western blot was performed from the immunoprecipitate using DRIL1 antibody at a dilution of 1:500.
DRIL1 Antibody

Western Blot: DRIL1 Antibody [NBP3-38452] -

Western Blot: DRIL1 Antibody [NBP3-38452] - Western blot analysis of lysates from K-562 cells using DRIL1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for DRIL1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DRIL1

DRIL1 encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.

Alternate Names

ARID domain-containing 3A, ARID domain-containing protein 3A, AT rich interactive domain 3A (BRIGHT- like), AT rich interactive domain 3A (BRIGHT- like) protein, AT rich interactive domain 3A (BRIGHT-like), AT-rich interactive domain-containing protein 3A, B-cell regulator of IgH transcription, BRIGHT, dead ringer-like 1, Dead ringer-like protein 1, DRIL1dead ringer-like 1 (Drosophila), DRIL3, DRX, E2F-binding protein 1, E2FBP1

Gene Symbol

ARID3A

Additional DRIL1 Products

Product Documents for DRIL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DRIL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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